Aurora A anticorps (C-Term)

N° du produit ABIN184572

Cet anticorps anti-Aurora A est un anticorps Chèvre Polyclonal détectant Aurora A dans WB, ELISA et IP. Adapté pour Humain.

Aperçu rapide pour Aurora A anticorps (C-Term) (ABIN184572)

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Aurora A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-Aurora A anticorps

Épitope: C-Term

Fonction: Aurora kinase A / AURKA

Séquence: QNKESASKQS

Specificité: Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 45 kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8 kDa according to NP_003591.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Aurora A

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Autre désignation: AURKA

Sujet: AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser

ID gène: 6790

NCBI Accession: NP_003591

Pathways: Cycle Cellulaire, Asymmetric Protein Localization